A novel Omega-class glutathione S-transferase gene in Apis cerana cerana: molecular characterisation of GSTO2 and its protective effects in oxidative stress
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چکیده
منابع مشابه
Molecular characterization and oxidative stress response of a cytochrome P450 gene (CYP4G11) from Apis cerana cerana.
Cytochrome P450 proteins, widely distributed multifunctional enzymes, are mainly involved in biosynthetic and degradative pathways of endogenous compounds and the detoxification of xenobiotics in insects. Moreover, these enzymes exhibit peroxidase-like activity, therefore they may be involved in protecting organisms against the toxicity of reactive oxygen species (ROS). In the present study, we...
متن کاملCharacterization and Mutational Analysis of Omega-Class GST (GSTO1) from Apis cerana cerana, a Gene Involved in Response to Oxidative Stress
The Omega-class of GSTs (GSTOs) is a class of cytosolic GSTs that have specific structural and functional characteristics that differ from those of other GST groups. In this study, we demonstrated the involvement of the GSTO1 gene from A. cerana cerana in the oxidative stress response and further investigated the effects of three cysteine residues of GSTO1 protein on this response. Real-time qu...
متن کاملStudy of the obp5 gene in Apis mellifera ligustica and Apis cerana cerana.
Apis mellifera ligustica and A. cerana cerana exhibit differences in olfactory sensitivity to odors from nectariferous plants and diseased broods. It is presumed that the differences in odorant-binding proteins (OBPs) between these 2 species contribute to their olfactory sensitivity. We compared the sequences, temporal expression pattern, and binding properties of the 2 OBP-encoding genes. We c...
متن کاملOxidative stress in Caenorhabditis elegans: protective effects of the Omega class glutathione transferase (GSTO-1).
To elucidate the function of Omega class glutathione transferases (GSTs) (EC 2.5.1.18) in multicellular organisms, the GSTO-1 from Caenorhabditis elegans (GSTO-1; C29E4.7) was investigated. Disc diffusion assays using Escherichia coli overexpressing GSTO-1 provided a test of resistance to long-term exposure under oxidative stress. After affinity purification, the recombinant GSTO-1 had minimal ...
متن کاملCharacterization of a Decapentapletic Gene (AccDpp) from Apis cerana cerana and Its Possible Involvement in Development and Response to Oxidative Stress
To tolerate many acute and chronic oxidative stress-producing agents that exist in the environment, organisms have evolved many classes of signal transduction pathways, including the transforming growth factor β (TGFβ) signal pathway. Decapentapletic gene (Dpp) belongs to the TGFβ superfamily, and studies on Dpp have mainly focused on its role in the regulation of development. No study has inve...
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ژورنال
عنوان ژورنال: Cell Stress and Chaperones
سال: 2013
ISSN: 1355-8145,1466-1268
DOI: 10.1007/s12192-013-0406-2